The governing body, along with the Gambling Commission, has appealed to the clubs for information about possible breaches of betting rules.
All clubs have been asked to remind players and officials of their responsibilities under the rules.
Sponsor Blue Square has suspended some betting markets for the division.
"Anyone with any information regarding possible breaches of these rules has been advised to come forward to The FA or to the Gambling Commission," said an FA statement.
The FA said it will not be making any further comment on this matter.
A spokesman for the league, which is in the sixth-tier of English football, added it was "inappropriate to make any comment at this stage of the investigation".
Alan Alger, spokesman for league sponsors Blue Square, said: "As always we will assist the authorities with their investigation."
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